Preparation and identification of monoclonal antibody against C1q/TNF-related protein 4
H Huang, X Wu, L Cao, L Wang - Monoclonal Antibodies in …, 2016 - liebertpub.com
H Huang, X Wu, L Cao, L Wang
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 2016•liebertpub.comCTRP4 (C1q/TNF-related protein 4) is a secreted cytokine homologous to adiponectin,
which plays an important role in immunity and metabolism. This study was designed to
generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice
immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate
hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs
were purified. The specificity and affinity were identified by Western blot and enzyme-linked …
which plays an important role in immunity and metabolism. This study was designed to
generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice
immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate
hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs
were purified. The specificity and affinity were identified by Western blot and enzyme-linked …
CTRP4 (C1q/TNF-related protein 4) is a secreted cytokine homologous to adiponectin, which plays an important role in immunity and metabolism. This study was designed to generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs were purified. The specificity and affinity were identified by Western blot and enzyme-linked immunosorbent assay, whereas CTRP4 endogenous expression was identified by immunofluorescence staining. These MAbs could be useful tools for basic research and potential clinical application.
Mary Ann Liebert