[HTML][HTML] Pit-1 and GATA-2 interact and functionally cooperate to activate the thyrotropin β-subunit promoter
DF Gordon, SR Lewis, BR Haugen, RA James… - Journal of Biological …, 1997 - ASBMB
The molecular determinants governing cell-specific expression of the thyrotropin (TSH) β-
subunit gene in pituitary thyrotropes are not well understood. The P1 region of the mouse
TSHβ promoter (− 133 to− 88) region interacts with Pit-1 and an additional 50-kDa factor at
an adjacent site that resembles a consensus GATA binding site. Northern and Western blot
assays demonstrated the presence of GATA-2 transcripts and protein in TtT-97 thyrotropic
tumors. In electrophoretic mobility shift assays, a comigrating complex was observed with …
subunit gene in pituitary thyrotropes are not well understood. The P1 region of the mouse
TSHβ promoter (− 133 to− 88) region interacts with Pit-1 and an additional 50-kDa factor at
an adjacent site that resembles a consensus GATA binding site. Northern and Western blot
assays demonstrated the presence of GATA-2 transcripts and protein in TtT-97 thyrotropic
tumors. In electrophoretic mobility shift assays, a comigrating complex was observed with …