[HTML][HTML] Association of excitatory amino acid transporters, especially EAAT2, with cholesterol-rich lipid raft microdomains: importance for excitatory amino acid …
MER Butchbach, G Tian, H Guo, CG Lin - Journal of Biological Chemistry, 2004 - ASBMB
In the present study, we investigated the role of membrane cholesterol in the function of
glutamate transporters. Depletion of membrane cholesterol by methyl-β-cyclodextrin
resulted in reduced Na+-dependent glutamate uptake in primary cortical cultures. Glial
glutamate transporter EAAT2-mediated uptake was more sensitive to this effect. Cell surface
biotinylation and immunostaining experiments revealed that the loss of cholesterol
significantly altered the trafficking of EAAT2 to the plasma membrane as well as their …
glutamate transporters. Depletion of membrane cholesterol by methyl-β-cyclodextrin
resulted in reduced Na+-dependent glutamate uptake in primary cortical cultures. Glial
glutamate transporter EAAT2-mediated uptake was more sensitive to this effect. Cell surface
biotinylation and immunostaining experiments revealed that the loss of cholesterol
significantly altered the trafficking of EAAT2 to the plasma membrane as well as their …