[CITATION][C] A suggested explanation for the paradoxically slow growth rate of basal‐cell carcinomas that contain numerous mitotic figures
JFR Kerr, J Searle - The Journal of pathology, 1972 - Wiley Online Library
JFR Kerr, J Searle
The Journal of pathology, 1972•Wiley Online LibraryMATERIALS AND METHODS Thirty consecutive surgically excised basal-cell carcinomas of
skin were examined by conventional histological techniques. Tissue from four of the tumours
was also processed for electron microscopy: 1 mm3 blocks were iixed for 2 hr in ice-cold 5
per cent. cacodylatebuffered glutaraldehyde solution, washed with buffer and post-fixed for
90 min. in ice-cold 1 per cent. cacodylate-buffered osmium tetroxide solution. After further
washing, the blocks were stained in 5 per cent. uranyl acetate solution for 30 min …
skin were examined by conventional histological techniques. Tissue from four of the tumours
was also processed for electron microscopy: 1 mm3 blocks were iixed for 2 hr in ice-cold 5
per cent. cacodylatebuffered glutaraldehyde solution, washed with buffer and post-fixed for
90 min. in ice-cold 1 per cent. cacodylate-buffered osmium tetroxide solution. After further
washing, the blocks were stained in 5 per cent. uranyl acetate solution for 30 min …
MATERIALS AND METHODS
Thirty consecutive surgically excised basal-cell carcinomas of skin were examined by conventional histological techniques. Tissue from four of the tumours was also processed for electron microscopy: 1 mm3 blocks were iixed for 2 hr in ice-cold 5 per cent. cacodylatebuffered glutaraldehyde solution, washed with buffer and post-fixed for 90 min. in ice-cold 1 per cent. cacodylate-buffered osmium tetroxide solution. After further washing, the blocks were stained in 5 per cent. uranyl acetate solution for 30 min., dehydrated in ethanol and embedded in Epon 812. 1-pm sections were stained with toluidine blue and studied with a
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